Peak At Science: 2022 December 27

Peak At Science 20221227
Open Access

Peak At Science: 2022 December 27

Absorption measurements of a cell monolayer relevant to phototherapy: Reduction of cytochrome c oxidase under near IR radiation

Phototherapy uses monochromatic light in the optical region of 600–1000 nm to treat in a non-destructive and non-thermal fashion various soft-tissue and neurological conditions. This kind of treatment is based on the ability of light red-to-near IR to alter cellular metabolism as a result of its being absorbed by cytochrome c oxidase. To further investigate the involvement of cytochrome c oxidase as a photoacceptor in the alteration of the cellular metabolism, we have aimed our study at, first, recording the absorption spectra of HeLa-cell monolayers in various oxygenation conditions (using fast multichannel recording), secondly, investigating the changes caused in these absorption spectra by radiation at 830 nm (the radiation wavelength often used in phototherapy), and thirdly, comparing between the absorption and action spectra recorded. The absorption measurements have revealed that the 710- to 790-nm spectral region is characteristic of a relatively reduced photoacceptor, while the 650- to 680-nm one characterizes a relatively oxidized photoacceptor. The ratio between the peak intensities at 760 and 665 nm is used to characterize the redox status of cytochrome c oxidase. By this criterion, the irradiation of the cellular monolayers with light at k = 830 nm (D = 6.3 · 10 3 J/m 2 ) causes the reduction of the photoacceptor. A similarity is established between the peak positions at 616, 665, 760, 813, and 830 nm in the absorption spectra of the cellular monolayers and the action spectra of the long-term cellular responses (increase in the DNA synthesis rate and cell adhesion to a matrix).


光療法は、6001000 nmの光学領域で単色光を使用して、非破壊的で非熱的な方法でさまざまな軟部組織と神経学的状態を治療します。 この種の治療は、シトクロムCオキシダーゼに吸収された結果、細胞代謝を変化させる軽い赤からdear IRの能力に基づいています。 細胞代謝の変化における光学受容体としてのシトクロムCオキシダーゼの関与をさらに調査するために、最初に、さまざまな酸素化条件(高速マルチチャネル記録を使用)におけるHeLa細胞単層の吸収スペクトルを記録することを目指しました。 、830 nm(光線療法でよく使用される放射波長)での放射によってこれらの吸収スペクトルに引き起こされる変化を調査し、第三に、記録された吸収スペクトルと作用スペクトルを比較します。 吸収測定により、710790 nmのスペクトル領域は比較的減少した光感受性の特徴であり、650680 nmは比較的酸化された光受容器を特徴づけることが明らかになりました。 760 nm665 nmでのピーク強度の比を使用して、シトクロムCオキシダーゼの酸化還元状態を特徴付けます。 この基準により、k = 830 nmd = 6.310 3 j/m 2)の光による細胞単層の照射は、光感受性の減少を引き起こします。 細胞単層の吸収スペクトルにおける616665760813、および830 nmのピーク位置と、長期の細胞応答の作用スペクトルの間に類似性が確立されます(DNA合成速度の増加と細胞接着の増加 マトリックス)。

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